Review



tm bicinchoninic acid bca protein assay kit  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher tm bicinchoninic acid bca protein assay kit
    Tm Bicinchoninic Acid Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tm+bca+protein+assay+kit/BCA+Protein+Assay+Kit/bio_rxiv__64898__2026__06__02__729508-150-11-19
    Average 99 stars, based on 1 article reviews
    tm bicinchoninic acid bca protein assay kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: A Step-by-Step Protocol for the Isolation of Aloe vera –Derived Extracellular Vesicles via Manual and Shear-Force Homogenization
    Article Snippet: Pierce TM RIPA lysis buffer (Thermo Scientific, catalog number: 89901) 4. .. Pierce TM BCA Protein Assay kit (Thermo Scientific, catalog number: 23225) a. .. Bovine serum albumin (BSA) ampules, 2 mg/mL (Thermo Scientific, catalog number: 23209) b. Reagent A (Thermo Scientific, catalog number: 23228) c. Reagent B (Thermo Scientific, catalog number: 1859078) 5.

    Article Title: Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
    Article Snippet: .. After removing cell debris by centrifugation at 11,000× g for 10 min, protein concentration of cell lysates was determined using the Pierce TM BCA protein assay kit according to manufacturer’s instructions (Thermo Scientific, 23227). ..

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets
    Article Snippet: .. The protein concentration of cell lysates was determined using the Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, #23225) following the manufactureŕs instructions. .. Cell lysates (40 μg protein per sample) were incubated with NuPAGE TM LDS Sample buffer (Thermo Fisher Scientific, Cat#NP0007) containing 10% β-Mercaptoethanol (Roth, Cat#4227.1) for 10 min at 70 °C.

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Cell pellets were resuspended in lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris–HCl, pH 8.0, 2 mM EDTA, pH 8.0), containing 1× PIC (Calbiochem, CA, USA) and 1× PhosSTOP (Roche) and 20 μM DUBi (Deubiquitylase Inhibitor; PR-619, Calbiochem, CA, USA) with gentle sonication 10 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with the Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4 °C with continuous rotation.

    Article Title: Tau oligomerization induces nuclear lamina invagination and chromatin remodeling in Alzheimer’s disease
    Article Snippet: .. BSA standards were prepared using the Pierce TM BCA Protein Assay Kit (#23,227, Thermo Fisher, USA). ..

    Article Title: Non-invasive Bdnf mRNA therapy improves cognition in ageing and Alzheimer’s mouse models
    Article Snippet: .. Total protein concentrations were determined using a Pierce TM BCA Protein Assay kit (ThermoFisher, #23225) according to the manufacturer’s instructions. .. A total of 20 μg protein per sample was loaded onto a 4-20% Tris-Glycine SDS-PAGE gel (Bio-Rad, #4561094), electrophoresed and transferred onto a nitrocellulose membrane (LI-COR, 926-31092).

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Supernatants were later pooled, and salt concentration was restored to 150 mM, and samples were sonicated 3 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4°C with continuous rotation.

    Article Title: TGF-β/SMAD4/14-3-3σ/TFEB axis promotes mesenchymal-epithelial transition and inhibits autophagy in colorectal cancer
    Article Snippet: Cells were washed with HBSS and lysed with RIPA lysis buffer containing mini protease inhibitor (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche). .. The cell lysate was sonicated for 5 s and then centrifuged at 13,000 rpm for 20 min at 4 °C to collect the supernatant containing proteins, which were then quantified according to the instructions of the Pierce TM BCA protein Assay Kit (Thermo Fisher Scientific). .. Protein samples were separated by electrophoresis on 10% or 12% SDS-PAGE gels and transferred to PVDF membranes (Millipore).

    Centrifugation:

    Article Title: Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
    Article Snippet: .. After removing cell debris by centrifugation at 11,000× g for 10 min, protein concentration of cell lysates was determined using the Pierce TM BCA protein assay kit according to manufacturer’s instructions (Thermo Scientific, 23227). ..

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Cell pellets were resuspended in lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris–HCl, pH 8.0, 2 mM EDTA, pH 8.0), containing 1× PIC (Calbiochem, CA, USA) and 1× PhosSTOP (Roche) and 20 μM DUBi (Deubiquitylase Inhibitor; PR-619, Calbiochem, CA, USA) with gentle sonication 10 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with the Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4 °C with continuous rotation.

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Supernatants were later pooled, and salt concentration was restored to 150 mM, and samples were sonicated 3 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4°C with continuous rotation.

    Protein Concentration:

    Article Title: Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity
    Article Snippet: .. After removing cell debris by centrifugation at 11,000× g for 10 min, protein concentration of cell lysates was determined using the Pierce TM BCA protein assay kit according to manufacturer’s instructions (Thermo Scientific, 23227). ..

    Article Title: Multifaceted immune resistance landscapes in human oligodendrocytes protect against cytotoxic T cells and are dysregulated in MS brain cell subsets
    Article Snippet: .. The protein concentration of cell lysates was determined using the Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, #23225) following the manufactureŕs instructions. .. Cell lysates (40 μg protein per sample) were incubated with NuPAGE TM LDS Sample buffer (Thermo Fisher Scientific, Cat#NP0007) containing 10% β-Mercaptoethanol (Roth, Cat#4227.1) for 10 min at 70 °C.

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Cell pellets were resuspended in lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris–HCl, pH 8.0, 2 mM EDTA, pH 8.0), containing 1× PIC (Calbiochem, CA, USA) and 1× PhosSTOP (Roche) and 20 μM DUBi (Deubiquitylase Inhibitor; PR-619, Calbiochem, CA, USA) with gentle sonication 10 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with the Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4 °C with continuous rotation.

    Article Title: K63-linked ubiquitylation of S2P–RNAPII regulates transcription in a DNAPK inter-dependent manner in response to double-strand breaks
    Article Snippet: Supernatants were later pooled, and salt concentration was restored to 150 mM, and samples were sonicated 3 × 30 s ON/30 s OFF in a Bioruptor Pico sonicator (Diagenode). .. Centrifugation at 13 000 rpm for 10 min was used to clarify the supernatant, and protein concentration was determined with Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA). .. Protein A/G PLUS-Agarose beads (sc-2003, Santa-Cruz, CA, USA) were washed extensively, and 0.5 mg of protein lysate was pre-cleared with 15 μl of beads 50% slurry for 1 h at 4°C with continuous rotation.

    Sonication:

    Article Title: TGF-β/SMAD4/14-3-3σ/TFEB axis promotes mesenchymal-epithelial transition and inhibits autophagy in colorectal cancer
    Article Snippet: Cells were washed with HBSS and lysed with RIPA lysis buffer containing mini protease inhibitor (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche). .. The cell lysate was sonicated for 5 s and then centrifuged at 13,000 rpm for 20 min at 4 °C to collect the supernatant containing proteins, which were then quantified according to the instructions of the Pierce TM BCA protein Assay Kit (Thermo Fisher Scientific). .. Protein samples were separated by electrophoresis on 10% or 12% SDS-PAGE gels and transferred to PVDF membranes (Millipore).



    Similar Products

    99
    Thermo Fisher tm bicinchoninic acid bca protein assay kit
    Tm Bicinchoninic Acid Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tm+bca+protein+assay+kit/BCA+Protein+Assay+Kit/bio_rxiv__64898__2026__06__02__729508-150-11-19
    Average 99 stars, based on 1 article reviews
    tm bicinchoninic acid bca protein assay kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Epizyme Inc omni easy tm bicinchoninic acid bca protein assay kit
    Omni Easy Tm Bicinchoninic Acid Bca Protein Assay Kit, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tm+bca+protein+assay+kit/assay+bca+kit+protein/pmc13207757-117-25-33
    Average 86 stars, based on 1 article reviews
    omni easy tm bicinchoninic acid bca protein assay kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher tm bca protein assay kit
    Tm Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tm+bca+protein+assay+kit/BCA+Protein+Assay+Kit/pmc13122179-114-21-26
    Average 99 stars, based on 1 article reviews
    tm bca protein assay kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher bca tm protein assay kit
    Bca Tm Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tm+bca+protein+assay+kit/BCA+Protein+Assay+Kit/bio_rxiv__64898__2026__04__16__718930-242-7-12
    Average 99 stars, based on 1 article reviews
    bca tm protein assay kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results